《植物生理学报》 2017, 53(4): 668-676

拟南芥COI1基因调控蔗糖响应的研究

黄瑞华, 古杰娜, 张盛春^*

华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广州510631

通信作者：张盛春；E-mail: sczhang@scnu.edu.cn

摘　要：

植物激素茉莉酸和糖在植物生长发育中起重要作用, 但两者之间的关系尚不清楚。本文研究了拟南芥茉莉酸受体基因COI1在植物响应无蔗糖反应中的功能。蔗糖能够诱导COI1基因表达, 与野生型植物相比, coi1突变体在无蔗糖培养基中能够正常生长, 主根增长, 蔗糖含量增加, 磷酸蔗糖合成酶基因SPSA1和SPSC以及生长素合成基因YACCA6和YUCCA8表达上调。结果表明COI1基因能够特异地在植物响应蔗糖中起重要调控作用。

关键词：拟南芥; COI1; 茉莉酸; 蔗糖响应; 生长素

收稿：2017-01-13   修定：2017-02-09

资助：国家自然科学基金(31370350)和广东省自然科学基金(2014A030313443)。

Study on Arabidopsis thaliana COI1 gene regulates plant response to sucrose

Huang Rui-Hua, Gu Jie-Na, Zhang Sheng-Chun^*

College of Life Sciences, South China Normal University; Guangdong Provincial Key Lab of Biotechnology for Plant Development, Guangzhou 510631, China

Corresponding author: Zhang Sheng-Chun; E-mail: sczhang@scnu.edu.cn

Abstract:

Plant phytohormone jasmonic acid (JA) and sugar play important roles in plant growth and development, however, their relationship has not been clearly demonstrated. The present study analyzed the function of Arabidopsis thaliana JA receptor gene COI1 on sucrose shortage response. The COI1 gene expression is up-regulated by sucrose. Compared to the wild type plants, coi1 mutant could grow without sucrose, had longer main root length, increased sucrose content, up-regulated expression levels of sucrose phosphate synthase genes SPSA1 and SPSC, as well as auxin biosynthesis genes YUCCA6 and YUCCA8. These results suggest that COI1 may play important roles in regulating plant response to sucrose specifically.

Key words: Arabidopsis thaliana; COI1; jasmonic acid; sucrose response; auxin